Immunotoxicity of acrylamide in female BALB/c mice.

Jin FANG; Chun Lai LIANG; Xu Dong JIA; Ning LI

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Immunotoxicity of acrylamide in female BALB/c mice.

Author: Jin FANG ^{1
,

2} ; Chun Lai LIANG ³ ; Xu Dong JIA ³ ; Ning LI ³
Author Information

1. Key Laboratory of Food Safety Risk Assessment, Ministry of Health, National Center for Food Safety Risk Assessment, Beijing 100021, China
2. National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
3. Key Laboratory of Food Safety Risk Assessment, Ministry of Health, National Center for Food Safety Risk Assessment, Beijing 100021, China.
Publication Type:Journal Article
Keywords: Acrylamide(ACR); Female BALB/c mice; Immunotoxicity
MeSH: Acrylamide; toxicity; Animals; Body Weight; drug effects; CD4-CD8 Ratio; Cytokines; blood; Female; Immunity, Cellular; drug effects; Immunity, Humoral; drug effects; Immunophenotyping; Immunotoxins; toxicity; Mice; Mice, Inbred BALB C; Organ Size; drug effects; Random Allocation; Spleen; drug effects; Thymus Gland; drug effects; Toxicity Tests
From: Biomedical and Environmental Sciences 2014;27(6):401-409
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the immunotoxicity of acrylamide (ACR) in female BALB/c mice.
METHODSA total of 200 female mice weighing 18-22 g were randomly divided into four clusters based on body weight, and each weight-based cluster included five groups (10 mice per group): negative control, positive control (cyclophosphamide), low, intermediate, and high dose ACR groups, and all the groups were administered ACR by gavage for 30 days. At the end of the study, the immunotoxicological effects of the ACR were evaluated through immunopathology, humoral immunity, cellular immunity, and non-specific immunity.
RESULTSThe terminal body weight, spleen and thymus weights, lymphocyte counts in the ACR-H group were decreased, pathological changes were observed in lymph glands, thymus and spleen. %T cells in blood lymphocytes were significantly increased in all ACR-treated groups, and a significant reduction of % natural killer(NK) cells and increase of %Th cells were observed in the ACR-H group. interleukin-6(IL-6), Concanavalin A(ConA)-induced splenocyte proliferation and serum half hemolysis value (HC50) were also significantly suppressed in the ACR-H group.
CONCLUSIONACR elicited an inhibitory effect on cellular and humoral immunity of mice after 30 day feeding.