Modification and evaluation of Brucella broth based Campylobacter jejuni transport medium.

Yao Bai; Sheng Hui Cui; Xiao XU; Feng Qin LI

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Modification and evaluation of Brucella broth based Campylobacter jejuni transport medium.

Author: Yao BAI ^{1
,

2} ; Sheng Hui CUI ³ ; Xiao XU ³ ; Feng Qin LI ⁴
Author Information

1. Key Laboratory of Food Safety Risk Assessment, National Health and Family Planning Commission, China National Center for Food Safety Risk Assessment, Beijing 100021, China
2. National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
3. National Institutes for Food and Drug Control, Beijing 100050, China.
4. Key Laboratory of Food Safety Risk Assessment, National Health and Family Planning Commission, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
Publication Type:Letter
MeSH: Bacteriological Techniques; Campylobacter jejuni; Culture Media
From: Biomedical and Environmental Sciences 2014;27(6):466-470
CountryChina
Language:English
Abstract: Reliable transport of Campylobacter jejuni isolates is critical to microbial epidemiology research, especially in developing countries without a good temperature control mailing system. Various factors, including oxygen, temperature, transport medium composition, could affect the survival of C. jejuni. In this study, the protective effects of different ingredients in C. jejuni transport media at 4 °C and 25 °C and under aerobic condition were quantitatively evaluated respectively. The results showed that enriched medium, supplementation with 5% blood and being kept at 4 °C could improve the viability of different C. jejuni strains during transport. In addition, supplementation with 25 mmol/L L-fucose in Wang's transport medium could significantly improve the survival of C. jejuni at both 4 °C and 25 °C. To the best of our knowledge, this is the first report to evaluate the protective effect of L-fucose in enriched C. jejuni transport medium which is feasible in developing countries without an effective cold chain mailing system. These data will be good reference for C. jejuni transport medium improvement in future.