Toxicity of multi-walled carbon nanotubes, graphene oxide, and reduced graphene oxide to zebrafish embryos.
- Author:
Xiao Tong LIU
1
;
Xi Yan MU
1
;
Xiao Li WU
1
;
Li Xuan MENG
1
;
Wen Bi GUAN
1
;
Yong Qiang MA
1
;
Hua SUN
2
;
Cheng Ju WANG
1
;
Xue Feng LI
1
Author Information
- Publication Type:Journal Article
- Keywords: Graphene Oxide; Multi-Walled Carbon Nanotubes; Reduced Graphene Oxide; Toxicity; Zebrafish Embryos
- MeSH: Animals; Embryo, Nonmammalian; Embryonic Development; drug effects; Female; Graphite; toxicity; Heart Rate; drug effects; Male; Movement; drug effects; Nanotubes, Carbon; toxicity; Oxides; toxicity; Toxicity Tests; Zebrafish
- From: Biomedical and Environmental Sciences 2014;27(9):676-683
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEThis study was aimed to investigate the toxic effects of 3 nanomaterials, i.e. multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO), and reduced graphene oxide (RGO), on zebrafish embryos.
METHODSThe 2-h post-fertilization (hpf) zebrafish embryos were exposed to MWCNTs, GO, and RGO at different concentrations (1, 5, 10, 50, 100 mg/L) for 96 h. Afterwards, the effects of the 3 nanomateria on spontaneous movement, heart rate, hatching rate, length of larvae, mortality, and malformations ls were evaluated.
RESULTSStatistical analysis indicated that RGO significantly inhibited the hatching of zebrafish embryos. Furthermore, RGO and MWCNTs decreased the length of the hatched larvae at 96 hpf. No obvious morphological malformation or mortality was observed in the zebrafish embryos after exposure to the three nanomaterials.
CONCLUSIONMWCNTs, GO, and RGO were all toxic to zebrafish embryos to influence embryos hatching and larvae length. Although no obvious morphological malformation and mortality were observed in exposed zebrafish embryos, further studies on the toxicity of the three nanomaterials are still needed.
