H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China.

Hong BO; Li Bo Dong; Ye Zhang; Jie Dong; Shu Mei Zou; Rong Bao Gao; Da Yan Wang; Yue Long Shu

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H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China.

Author: Hong BO ^{1
,

2} ; Li Bo DONG ¹ ; Ye ZHANG ¹ ; Jie DONG ¹ ; Shu Mei ZOU ¹ ; Rong Bao GAO ¹ ; Da Yan WANG ¹ ; Yue Long SHU ¹ ;
Author Information

1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention
2. Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China.
Publication Type:Journal Article
Keywords: H5N1; Influenza; Pre-pandemic; Vaccine strains
MeSH: Animals; Chick Embryo; Chickens; China; Hemagglutinin Glycoproteins, Influenza Virus; genetics; metabolism; Influenza A Virus, H5N1 Subtype; immunology; Influenza Vaccines; immunology; Influenza in Birds; prevention & control; virology; Neuraminidase; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Vaccines, Synthetic; immunology
From: Biomedical and Environmental Sciences 2014;27(10):763-769
CountryChina
Language:English
Abstract:
OBJECTIVETo prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China.
METHODSRecombinant viruses were rescued using reverse genetics. Neuraminidase (NA) and hemagglutinin (HA) segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpolI vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embryonate survival and antigenicity were compared with those of the respective wild-type viruses.
RESULTSThe 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chicken embryo survival and trypsin-dependent characteristics.
CONCLUSIONThe 4 recombinant viruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines.