Stable Silver Nanoparticles Synthesis by Citrus Sinensis (Orange) and Assessing Activity Against Food Poisoning Microbes.

Author: Arooj NAILA ¹ ; Dar NADIA ² ; Qadir Samra ZAHOOR ¹
Author Information

1. Institute of Biochemistry and Biotechnology, Quaid-i-Azam Campus University of the Punjab, Lahore 54590, Pakistan.
2. Department of Biology, Jinnah Degree College for Women, Mozang Lahore 54590, Pakistan.
Publication Type:Letter
MeSH: Anti-Bacterial Agents; pharmacology; Bacillus subtilis; drug effects; Bacteria; drug effects; Citrus sinensis; chemistry; Escherichia coli; drug effects; Foodborne Diseases; prevention & control; Metal Nanoparticles; analysis; Plant Extracts; pharmacology; Shigella; drug effects; Silver; pharmacology; Staphylococcus aureus; drug effects
From: Biomedical and Environmental Sciences 2014;27(10):815-818
CountryChina
Language:English
Abstract: Silver nanoparticles are considered as good antimicrobial agent. AgNPs were synthesized by mixing silver nitrate solution with citrus sinesis extract for 2 h at 37 °C and analyzed by UV-visible spectra, SEM, XRD, and FTIR. AgNPs were tested against B. subtilis, Shigella, S. aureus, and E. coli. Minimum inhibitory concentration of AgNPs was 20 µg/mL for B. subtilis and Shigella and 30 µg/mL for S. aureus and E. coli. Antibiofilm activity (80% to 90%) was observed at 25 µg/mL. AgNPs were stable for five months with sustained antimicrobial activity. Biosynthesized AgNPs can be used to inhibit food poisoning microbial growth.