Study on inhibitory effects of c9, t11-conjugated linoleic acid on migration of human gastric carcinoma cell line via cyclooxygenase-2 pathway.
- Author:
Jing-Shu ZHANG
1
;
Qi WAN
;
Bing-Qing CHEN
;
Yan-Mei YANG
;
Yan-Hui GAO
;
Wen-Guang SUN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Movement; drug effects; physiology; Cyclooxygenase 2; metabolism; Cyclooxygenase 2 Inhibitors; pharmacology; Humans; Linoleic Acid; metabolism; pharmacology; Neoplasm Invasiveness; Stomach Neoplasms; metabolism; pathology; Tumor Cells, Cultured
- From: Chinese Journal of Preventive Medicine 2007;41(6):471-474
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibitory effects of c9, t11-conjugated linoleic acid (c9, t11-CLA) on migration of human gastric carcinoma cell line (SGC-7901) via cyclooxygenase-2 (COX-2) pathway.
METHODSAfter inhibiting COX-2 activity by 100 micromol/L COX-2 inhibitor NS-398 in SGC-7901 cell, we treated SGC-7901 cells with c9, t11-CLA at a concentration of 200,100, 50, 25 micromol/L for 24 h, respectively. Using reconstituted basement membrane invasion, adhesion, chemotaxis assays, we detected the effect of c9, t11-CLA and COX-2 on the cell migration.
RESULTSCompared to NS-398 group, 200, 100 micromol/L c9, t11-CLA significantly suppressed SGC-7901 cells invading into the reconstituted basement membrane (F = 14.309, P = 0.000; F = 19.005, P = 0.000). 200 micromol/L c9, t11-CLA significantly inhibited SGC-7901 cells adhering to laminin, fibronectin and Matrigel (F = 3.063, P = 0.021; F = 6.692, P = 0.001; F = 11.999, P = 0.000). The chemotaxis of SGC-7901 cells and inhibitory frequency were significantly decreased in the 200 micromol/L c9, t11-CLA group (F = 1.380, P = 0.276).
CONCLUSIONc9, t11-CLA inhibits invasion, adhesion and chemotaxis of SGC-7901 cells, and the COX-2 plays an important role in the process. [ Key words]
