Application of pulse-field gel electrophoresis analysis in source-tracking of food-borne disease caused by Vibrio parahaemolyticus.

Author: Zhi-Ai DENG ¹ ; Xiao-Quan LI ; Yu-Shan HU ; Jun-Hua LIU ; Xin-Qiang ZHANG ; Yun-Wan LIN ; Zi-Yao MO
Author Information

1. Guangzhou Center for Disease Control and Prevention, Guangzhou 510080, China.
Publication Type:Journal Article
MeSH: Bacterial Typing Techniques; methods; China; Electrophoresis, Gel, Pulsed-Field; methods; Foodborne Diseases; microbiology; Humans; Vibrio parahaemolyticus; classification; genetics; isolation & purification
From: Chinese Journal of Preventive Medicine 2008;42(1):36-38
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo apply pulse-field gel electrophoresis analysis(PFGE) in analysing a case of food poisoning caused by Vibrio parahaemolyticus.
METHODSPFGE using restriction enzyme Not I was employed in molecular subtyping of thirty strains of V. parahaemolyticus isolated from a case of food poisoning in Guangzhou city and PFGE patterns were analyzed by using BioNumerics Version 4.0 software to perform cluster analysis. Pattern profiles were compared by using the Dice coefficient and unweighted pair group method with arithmetic averages (UPGMA).
RESULTSThirty strains were of the same type of pulsotype.
CONCLUSIONSMolecular subtyping by PFGE might disclose the epidemiological relationships of the strains from humans, food and the environment, giving a strong molecular epidemiological evidence and a support for the source-tracking of outbreak events.