Effects of prenatal taurine on mRNA expression of PKA CREB signal pathway and glial cell line derived neurotrophic factor in fetal rat brains of intrauterine growth restriction.
- Author:
Hui CHEN
1
;
Jian LI
;
Jing LIU
;
Li LIU
;
Na LIU
;
Yi-Zhi SONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; drug effects; Cyclic AMP Response Element-Binding Protein; genetics; physiology; Cyclic AMP-Dependent Protein Kinases; genetics; physiology; Female; Fetal Growth Retardation; metabolism; Fetus; drug effects; Glial Cell Line-Derived Neurotrophic Factor; genetics; Male; Pregnancy; RNA, Messenger; analysis; Rats; Rats, Sprague-Dawley; Signal Transduction; drug effects; Taurine; pharmacology
- From: Chinese Journal of Contemporary Pediatrics 2009;11(11):923-926
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study examined the effects of prenatal application of taurine on mRNA expression of protein kinase A cAMP response element binding protein (PKA-CREB) signal pathway and glial cell line derived neurotrophic factor (GDNF) in fetal rat brains of intrauterine growth restriction (IUGR).
METHODSPregnant rats were randomly divided into 4 groups: normal control, IUGR model, low dose (100 mg/kg x d) and high dose (300 mg/kg x d) taurine treatment IUGR (n = 5 each). IUGR was induced by food restriction throughout pregnancy. PKA, CREB and GDNF mRNA expression in brains of newborn rats was detected by reverse transcription polymerase chain reaction (RT-PCR).
RESULTSPKA, CREB and GDNF mRNA expression in the IUGR model group was significantly higher than that in the normal control group (p<0.05). Compared with the IUGR model group, mRNA expression of PKA and CREB in both the low dose and high dose taurine treatment groups increased significantly (p<0.05); GDNF mRNA expression in the high dose taurine treatment group also increased significantly (p<0.01).
CONCLUSIONSTaurine can increase mRNA expression of PKA, CREB and GDNF in fetal rat brains of IUGR. This suggests that prenatal application of taurine may increase neurogenesis of the central nervous system and endogenous secretion of neurotrophic factors, thus providing neuroprotective effects.
