Experimental study on the adhesion, migration and three-dimensional growth of Schwann cells on absorbable biological materials.
- Author:
Guang-lin WANG
1
;
Wei LIN
;
Zhi-ming YANG
;
Fu-xing PEI
;
Lei LIU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion; Cell Division; Cell Movement; Humans; Lactic Acid; Polyglycolic Acid; Polymers; Schwann Cells; physiology; Tissue Engineering
- From: Chinese Journal of Traumatology 2003;6(4):209-212
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the adhesion, migration and three-dimentional growth of Schwann cells on PLA (polylactic acid) nonspinning fibre cloth and polyglycolic/polylactic acid (PLGA) fibres.
METHODSSchwann cells/ECM gel solution and PLA nonspinning fibre cloth and PLGA fibres pretreated by collagen, polylysine and ECM were co-cultured. Then the migration and three-dimensional growth of Schwann cells on the fibres were observed under phase contrast microscope and laser scanning confocal microscope.
RESULTSSchwann cell/ECM solution was compounded with PLA nonspinning fibre cloth. With formation of gel, most Schwann cells resided in the fibre net holes, and adhered to the fibres to form a multiplayer-arranged Schwann cell column like Büngner band. Schwann cells could adhere to PLGA fibres and grew and migrated along the fibres. ECM gel could significantly increase the adhering and migrating cell number.
CONCLUSIONSECM gel can facilitate the adhesion, growth and migration of Schwann cells on the seteroframe. It is a good integrating material for tissue engineering bioartificial nerve.
