Effects of basic fibroblast growth factor on biological characteristics of osteoblasts.
- Author:
Hui ZENG
1
;
Jing-yuan DU
;
Qi-xin ZHENG
;
De-yu DUAN
;
Yong LIU
;
Ao XIONG
;
Bin KANG
;
Guo-ping LIU
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Animals; Cells, Cultured; Fibroblast Growth Factor 2; pharmacology; Osteoblasts; drug effects; metabolism; Proliferating Cell Nuclear Antigen; analysis; RNA, Messenger; analysis; Rats; Rats, Sprague-Dawley; Transforming Growth Factor beta; genetics; Transforming Growth Factor beta1
- From: Chinese Journal of Traumatology 2003;6(4):229-233
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo elucidate the effects of exogenous basic fibroblast growth factor (bFGF) on biological characteristics of rat osteoblasts cultured in vitro.
METHODSThe osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF (5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 (TGF-beta(1)) was detected to observe the effects of bFGF on growth and differentiation of osteoblasts.
RESULTSbFGF (5-50 ng/ml) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-beta(1) mRNA increased significantly, but the intracellular ALP content decreased.
CONCLUSIONSbFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-beta(1), but cannot promote the differentiation of osteoblasts.
