Effect of decoy-oligodeoxynucleotides on expression of inflammation mediators in pMPhi cells from rats.
- Author:
Hua GUO
1
;
Hua-ping LIANG
;
Feng-lin LU
;
Xiang XU
;
Bin AN
;
Tian-zhi HAO
;
Fu-lLong WANG
;
Wen-jun YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Base Sequence; Cells, Cultured; Dose-Response Relationship, Drug; Female; Inflammation Mediators; metabolism; Interleukin-10; metabolism; Interleukin-6; metabolism; Male; Molecular Sequence Data; NF-kappa B; drug effects; metabolism; Oligodeoxyribonucleotides; pharmacology; RNA, Messenger; analysis; Random Allocation; Rats; Rats, Wistar; Reference Values; Reverse Transcriptase Polymerase Chain Reaction; methods; Sensitivity and Specificity; Tumor Necrosis Factor-alpha; drug effects; metabolism
- From: Chinese Journal of Traumatology 2003;6(6):363-369
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the effect of decoy-oligodeoxynucleotides (decoy-ODNs) in dumbbell shape with the oligodeoxynucleotide sequence similar to nuclear factor kappa B (NF-kappaB) cis-elements on expression of inflammation mediators in pMPhi cells from rats.
METHODSWith carriers of cationic liposomes, decoy-ODNs were transfected into pMPhi cells of rats. Then the inhibiting effects of the decoy-ODNs on tumor necrosis factor alpha (TNFalpha), interleukin-6 (IL-6) and IL-10 were analyzed.
RESULTSDecoy-ODNs could decrease the expression of TNFalpha and IL-6 in dose-dependent fashion but had weaker inhibiting effect on IL-10.
CONCLUSIONSDecoy-ODNs targeting NF-kappaB can decrease the expression of inflammatory mediators in pMPhi cells from rats.
