Pretreatment effect of adenosine on activation of NF-kappaB and level of TNF-alpha during myocardial ischemia and reperfusion in rats.
- Author:
Jian-juan KE
1
;
Yan-lin WANG
;
Jian-guo LI
;
Jing WU
;
Xiao-bo FENG
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine; pharmacology; Analysis of Variance; Animals; Blotting, Western; Confidence Intervals; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Male; Myocardial Ischemia; therapy; Myocardial Reperfusion; methods; Myocardial Reperfusion Injury; prevention & control; NF-kappa B; analysis; drug effects; Random Allocation; Rats; Rats, Sprague-Dawley; Reference Values; Sensitivity and Specificity; Tumor Necrosis Factor-alpha; analysis; drug effects
- From: Chinese Journal of Traumatology 2004;7(1):25-27
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the pretreatment effect of adenosine on NF-kappaB nuclear activity in ischemia/reperfusion myocardium in rats.
METHODSEighteen healthy male S-D rats were randomly divided into three groups. Group I was the control group. The other two groups were subjected to 30 minutes of ischemia, followed by 2 hours of reperfusion. In group III, adenosine was given 40 microg.kg(-1).min(-1) 30 minutes before coronary artery occlusion. The NF-kappaB in nuclear was extracted and measured with western blot analysis. TNF-alpha levels in myocardium were measured with enzyme-linked immunosorbent assay (ELISA) system. All the data were recorded with mean +/- SEM, differences at the 95% confidence level were considered significant.
RESULTSNF-kappaB activity in the nucleus significantly increased after ischemia/reperfusion and TNF-alpha levels changed. Adenosine significantly inhibited NF-kappaB activity in nucleus, and concomitantly decreased the level of TNF-alpha in myocardium.
CONCLUSIONSAdenosine modulation of NF-kappaB activation may be the cellular molecular mechanism of decreasing of TNF-alpha. The cardioprotective action of adenosine may be involved in the differential modulation of NF-kappaB activation during myocardial ischemia/reperfusion.
