Coculture of elongated neuron axon with poly (D,L-lactide-co-glycolide) biomembrane in vitro.
- Author:
Biao CHENG
1
;
Zheng-rong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Axons; drug effects; transplantation; Coculture Techniques; Humans; Immunohistochemistry; Lactic Acid; Polyglycolic Acid; Polymers; Tissue Engineering; methods
- From: Chinese Journal of Traumatology 2004;7(4):217-219
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo elongate human nerve axon in culture and search for suitable support matrices for peripheral nervous system transplantation.
METHODSHuman embryo cortical neuronal cells, seeded on poly (D,L-lactide-co-glycolide) (PLGA) membrane scaffolds, were elongated with a self-made neuro-axon extending device. The growth and morphological changes of neuron axons were observed to measure axolemmal permeability after elongation. Neurofilament protein was stained by immunohistochemical technique.
RESULTSHuman embryo neuron axon could be elongated and cultured on the PLGA membrane and retain their normal form and function.
CONCLUSIONSThree dimensional scaffolds with elongated neuron axon have the basic characteristics of artificial nerves, indicating a fundemental theory of nerve repair with elongated neuron axon.
