Preparation and characterization of specific monoclonal antibodies against hexon of HAdV 3.
- Author:
Rong ZHOU
1
;
Hui-Ying SHENG
;
Xin-Gui TIAN
;
Chang-Bing WANG
;
Si-Tang GONG
;
Qiao-Lian CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviruses, Human; chemistry; immunology; Animals; Antibodies, Monoclonal; biosynthesis; immunology; Antibodies, Viral; biosynthesis; immunology; Blotting, Western; Capsid Proteins; biosynthesis; genetics; immunology; Enzyme-Linked Immunosorbent Assay; Escherichia coli; genetics; Humans; Hybridomas; secretion; Mice; Mice, Inbred BALB C; Recombinant Proteins; biosynthesis; immunology
- From: Journal of Southern Medical University 2008;28(6):1008-1010
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo obtain the monoclonal antibody against hexon protein of human adenovirus.
METHODSBALB/c mice were immunized with purified recombinant hexon protein, and the spleen cells of the mice were isolated and fused with myloma cells. Four hybridoma cell strains were screened by indirect ELISA and cultured, and the sensitivity, specificity and virus neutralizing activity were analyzed with ELISA, Western blotting and neutralizing test.
RESULTSThe mouse ascites produced by these hybridoma cells contained specific monoclonal antibodies against hexon protein of human adenovirus as identified by ELISA and Western blot, and the antibody generated by 4C6 strain showed human adenovirus type 3-neutralizing activity.
CONCLUSIONThe monoclonal antibodies against hexon protein with high specificity have been successfully obtained, and these antibodies can be useful in developing assays for early diagnosis of HAdV3 infection and also in study of therapeutic drugs of the infection.
