High-performance liquid chromatography-mass spectrometry for determining olmesartan in human plasma.
- Author:
Shi-Heng CHEN
1
;
Cui-Fang WU
;
Ben-Mei CHEN
;
Qi PEI
;
Hong-Yi TAN
;
Li YANG
;
Guo-Ping YANG
Author Information
- Publication Type:Journal Article
- MeSH: Angiotensin II Type 1 Receptor Blockers; blood; Calibration; Chromatography, High Pressure Liquid; methods; Humans; Imidazoles; blood; Mass Spectrometry; methods; Reproducibility of Results; Tetrazoles; blood
- From: Journal of Southern Medical University 2008;28(6):1104-1105
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a simple and rapid HPLC-MS method for determining the contents of olmesartan in human plasma.
METHODSPlasma were precipitated with trifluoroacetic acid, then analyzed on an HyPurity C(18) column (150 mm 2.1 mm, 5 microm). Samples at 40 degrees celsius;. The mobile phase consisted of water-methanol- acetonitrile(14:60:26) with a flow rate of 0.22 ml/min.
RESULTSThe lower limit of qualification was 25 microg/L. The calibration curve was linear over the range of 25-3200 microg/L (r=0.9998), with the intra-day and inter-day RSD less than 15%.
CONCLUSIONThe method is sensitive, rapid and suitable for the study of pharmacokinetics and bioavailability of olmesartan.
