Cloning of Rcet3, a novel gene related to family 2 cystatins.
- Author:
Yang XIANG
1
;
Shou-chang ZOU
;
Xue-ling OU
;
Ming-qian ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Cystatins; genetics; DNA, Complementary; chemistry; genetics; Gene Expression Profiling; Male; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Testis; metabolism
- From: Journal of Southern Medical University 2008;28(7):1151-1153
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone the full-length Rcet3 gene, a novel gene related to family 2 cystatins, from mouse testis or other tissues.
METHODSRcet3 gene was cloned using digital differential display (DDD) and RT-PCR was performed for cloning the full-length Rcet3 gene from adult mouse testis cDNA library with sequence analysis.
RESULTSRcet3 cDNA was 610 bp in length, consisting of 4 exons to encode a protein with 140 amino acid residues. The encoded protein contained a potential signal peptide and a cystatin domain, but lacked critical consensus site important for cysteine protease inhibition. These characteristics could be seen in the Cres subgroup related to the family 2 cystatins. Rcet3 was specifically expressed in adult mouse testis, epididymis and the cerebrum, but at higher levels in the testis than in the epididymis and cerebrum.
CONCLUSIONRcet3 may be a new member of Cres subgroup of family 2 cystatins.
