Expression of osteopontin mRNA in oral squamous cell carcinoma and normal oral mucosa.
- Author:
Xiao-zhi LU
1
;
Xue-min YIN
;
Yuan-yong FENG
;
Li-qiong DUAN
;
Wan-tao CHEN
;
Chen-ping ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Carcinoma, Squamous Cell; genetics; pathology; Female; Gene Expression Regulation, Neoplastic; Humans; Lymphatic Metastasis; Male; Middle Aged; Mouth Mucosa; metabolism; pathology; Mouth Neoplasms; genetics; pathology; Osteopontin; genetics; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Journal of Southern Medical University 2008;28(7):1165-1167
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate osteopontin (OPN) mRNA expression in oral squamous cell carcinoma (OSCC) and normal oral mucosa tissues.
METHODSDifferential OPN gene expression were detected in 30 cancerous tissues and their paired normal tissues using real-time reverse transcription-PCR (real-time RT-PCR), and the data were analyzed statistically.
RESULTSReal-time RT-PCR results demonstrated that the relative expression level of OPN mRNA in the cancerous tissues were significantly higher than that in paired normal samples (4.17-/+0.51 vs 0.97-/+0.12, P<0.001), showing a 4.3-fold up-regulation. In the 30 OSCC specimens, OPN mRNA expression in the OSCC of histological grades I showed a 3.1-fold down-regulation, significantly lower than the expression in grade II/III tumors (2.16-/+0.17 vs 6.80-/+0.72, P<0.05); its expression was significantly lower in early stage than in advanced stage OSCCs (2.34-/+0.17 vs 4.73-/+0.35, P<0.05). In cases of cervical lymph node metastasis, the expression was significantly higher than that in cases without lymphatic metastasis (6.38-/+0.56 vs 2.89-/+0.32, P<0.05).
CONCLUSIONOPN mRNA overexpression may play an important role in OSCC carcinogenesis and can be a potential target for OSCC therapy.
