Immunological activity of Streptomyces polysaccharide in normal and immunosuppressed mice.
- Author:
Xiao-yun WEN
1
;
Chao SHI
;
Shu-guang WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CD4-Positive T-Lymphocytes; immunology; Female; Immunocompromised Host; immunology; Lymphocyte Culture Test, Mixed; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Polysaccharides; immunology; Spleen; cytology; Streptomyces; chemistry; immunology; T-Lymphocytes; cytology; immunology
- From: Journal of Southern Medical University 2008;28(7):1168-1171
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the immunological activity of Streptomyces polysaccharide (SMP) on normal and immunosuppressed mice.
METHODSThe effect of SMP on the proliferating activity of normal mouse splenocytes was tested in the mixed lymphocyte culture, and the changes of peripheral blood T lymphocytes were evaluated with acid a-naphthyl acetate esterase (ANAE) method. The ratio of Lyt2+ and L3T4+ T cell subsets was measured by flow cytometry.
RESULTSSMP stimulated obvious proliferation of mixed lymphocytes, showed protective effects on T lymphocyte and increased the ratio of Lyt2+ and L3T4+ cell subsets to nearly normal level in immunosuppressed mice.
CONCLUSIONSSMP can regulate the immune function in mice.
