Inhibitory effects of immunotargeting of Chinese cobra cytotoxin and iodine-131 against nasopharyngeal carcinoma cells in vitro.
- Author:
Yong-sheng ZHAO
1
;
Hui-ling YANG
;
Chang-zheng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; pharmacology; Antineoplastic Agents; pharmacology; Cell Line, Tumor; Cell Proliferation; drug effects; Cytotoxins; pharmacology; Elapid Venoms; pharmacology; Humans; Immunoconjugates; pharmacology; Iodine Radioisotopes; pharmacology; Nasopharyngeal Neoplasms; pathology
- From: Journal of Southern Medical University 2008;28(7):1235-1236
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore a new specific therapy of nasopharyngeal carcinoma (NPC) using an anti-nasopharyngeal carcinoma (NPC) monoclonal antibody BAC5 conjugate with Chinese cobra (CT) and iodine-131(131I).
METHODSBAC5 was labeled with 131I by chloramine-T method, CT was labeled with 125I using iodogen method, and BAC5 and 125I-CT were conjugated by SPDP method. The inhibitory effect of the conjugate on cultured human NPC CNE2 cells was observed using MTT assay.
RESULTSThe IC50 of 125I-CT-BAC5 conjugate was 9.17x10(-8) mol/L, and that of 131I-BAC5 was 5.83x10(8) Bq/L, and their combined administration showed obvious inhibitory effect on the NPC cells.
CONCLUSIONBoth 125I-CT-BAC5 and 131I-BAC5 have obvious inhibition effects against NPC cells, but their combined use shows stronger effects.
