Construction and identification of Ksp-cadherin-Gpx1-Klk1 expression vector.
- Author:
Li-yi XIE
1
;
Wu-jun XUE
;
He-li XIANG
;
Sun-kai MA
Author Information
- Publication Type:Journal Article
- MeSH: Cadherins; genetics; Cloning, Molecular; Genetic Therapy; methods; Genetic Vectors; genetics; Glutathione Peroxidase; genetics; Humans; Kallikreins; genetics; Kidney; metabolism; Promoter Regions, Genetic; genetics
- From: Journal of Southern Medical University 2008;28(8):1327-1330
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a Gpx1 and klk1 recombinant vector containing the kidney-specific promoter Ksp-cadherin.
METHODSHuman Gpx1, Klk1 and Ksp-cadherin cDNAs were amplified with PCR and inserted in a stepwise manner into the expressive vector pIRES-EGFP to construct the recombinant vector Ksp-cadherin-Gpx1-Klk1. The constructed vector was verified with restriction enzyme digestion and sequence analysis.
RESULTS AND CONCLUSIONThe recombinant expression vector Ksp-cadherin-Gpx1-Klk1 was constructed and identified successfully, which provides a potent tool for preparing transgenic animals to investigate gene therapy for ischemia-reperfusion injury in kidney transplantation.
