Isolation of the capsid protein gene of maize dwarf mosaic virus and its transformation in maize.
- Author:
Xiao-Hong LIU
1
;
Hong-Wei ZHANG
;
Xin LIU
;
Xin-Jie LIU
;
Zhen-Bo TAN
;
Ting-Zhao RONG
Author Information
1. Beijing Agro-biotechnology Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089, China.
- Publication Type:Journal Article
- MeSH:
Capsid Proteins;
genetics;
Cloning, Molecular;
Mosaic Viruses;
genetics;
Plant Diseases;
genetics;
virology;
Plants, Genetically Modified;
Transfection;
Zea mays;
genetics;
virology
- From:
Chinese Journal of Biotechnology
2005;21(1):144-148
- CountryChina
- Language:Chinese
-
Abstract:
The MDMV (Maize Dwarf Mosaic Virus, MDMV) CP (Coat Protein, CP) gene was cloned by RT-PCR method and introduced into the embryonic calli derived from immature embryos of elite inbred 18-599hong and 18-599bai via particle bombardment. Bombarded calli were selected on selection medium containing 5-10 mg/L (PPT) Bialaphos. From resistant calli, 79 plantlets were regenerated. 18 of 79 were grown and harvested. The results of Southern blotting and PCR analysis demonstrated that MDMV CP have been integrated into the genome of the transgenic plants. PCR-positive progeny plants were artificially inoculated with MDMV strain B, and the average chlorosis of the functional leaves of each plant was investigated. The typical symptoms were observed from the leaves of the control inbreds. while, the presence of the MDMV CP gene provided resistance to inoculation with MDMV strain B.
