Effects of nutrient conditions and fed-batch culture on CoQ10 production by Rhizobium radiobacter WSH2601.
- Author:
Wu ZU-FAN
1
;
Guo-Cheng DU
;
Jian CHEN
Author Information
1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036, China.
- Publication Type:Journal Article
- MeSH:
Agrobacterium tumefaciens;
growth & development;
metabolism;
Culture Media;
metabolism;
Fermentation;
physiology;
Glucose;
metabolism;
Industrial Microbiology;
methods;
Sucrose;
metabolism;
Ubiquinone;
analogs & derivatives;
biosynthesis
- From:
Chinese Journal of Biotechnology
2003;19(2):212-216
- CountryChina
- Language:Chinese
-
Abstract:
The effects of the variables, including the concentrations of glucose, sucrose, corn steep liquor (CSL) and peptone, and the conditions of fed-batch culture, on CoQ10 fermentation by Rhizobium radiobacter WSH2601 were assessed. The results showed that the optimum concentrations of glucose, sucrose, CSL and peptone were 30 g/L, 40 g/L, 11 g/L and 16 g/L respectively. Addition of CSL and tomato juice stimulated the cell growth. CSL, L-methionine and isopentyl alcohol efficiently increased the biosynthesis of CoQ10. In a 7L fermentor, the fed-batch culture improved both cell growth and CoQ10 production compared to a batch culture; and suitable mixed feeding of CSL and sucrose enhanced CoQ10 yield to 52.4 mg/L. The DCW reached 26.4 g/L, an increase of 53% in comparison to that without feeding, and an increase of 24% to that feeding with sucrose only. The C/B value reached 2.38 mg/g(DCW), representing an increase of 33% to that of without feeding, and an increase of 26% to that of feeding with sucrose only.
