Sequencing and bioinformatic analysis of NRDRiso cDNA.
- Author:
Jing DU
1
;
Ge-Fei LIU
;
Gui-Ling WANG
;
Xiao-Lin XU
;
Bo WANG
;
Li ZHU
Author Information
1. Department of Biochemistry, Shenyang Medical College, Shenyang, 110034, China. Dujing1106@sohu.com
- Publication Type:Journal Article
- MeSH:
Alcohol Oxidoreductases;
chemistry;
genetics;
Amino Acid Sequence;
Animals;
Base Sequence;
Chromosome Mapping;
Computational Biology;
DNA, Complementary;
chemistry;
Humans;
Isoenzymes;
genetics;
Liver;
enzymology;
Mice;
Molecular Sequence Data;
NADP;
metabolism;
Open Reading Frames;
Sequence Analysis, DNA
- From:
Chinese Journal of Biotechnology
2004;20(4):520-525
- CountryChina
- Language:Chinese
-
Abstract:
This study describes the cDNA sequencing and the bioinformatic analysis of a novel NADP(H)-dependent retinol dehydrogenase/reductases isoform (NRDRiso). Based upon the concensus sequences of human and mouse NRDR coding region, we have identified a short 377 bp RT-PCR product from human liver tissue. The cDNA sequence of a NRDR isoform was then isolated using RACE approach and its sequence was analysed. The full-length cDNA is 1,003bp in length and was submitted to GenBank as NADP-dependent retinol dehydrogenase/reductase short isoform (NRDRiso). The open reading frames of NRDRiso cDNA is 525 bp.
