The expression of growth hormone release factor gene carried with PLGA microspheres in muscle tissue and its effect on mouse growth.
- Author:
Xiao-Hui REN
1
;
Yong-Liang ZHANG
;
Song-Cai LIU
;
Jian-Wei DAI
Author Information
1. Department of Biochemistry, Changchun University of Agricultural and Animal Sciences, Changchun 130062, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Body Weight;
DNA;
administration & dosage;
Growth;
Growth Hormone-Releasing Hormone;
genetics;
Lactic Acid;
administration & dosage;
Male;
Mice;
Microspheres;
Muscle, Skeletal;
metabolism;
Plasmids;
Polyglycolic Acid;
administration & dosage;
Polymerase Chain Reaction
- From:
Chinese Journal of Biotechnology
2004;20(4):615-618
- CountryChina
- Language:Chinese
-
Abstract:
Injectable sustained-release pcDNA3-GRF (1-32) microspheres were prepared by double emulsion-in liquid evaporation process,using biodegrable poly lactic-co-glycolic acid as carrier. The enrapment efficiency, mean particle size, drug content thus prepared were 69%, 2.20 microm, 8% and 70% respectively. The result of transfection in vivo showed that after 30 days, accumulative increased body weights on the group injected with pcDNA3-GRF (1-32) microspheres was significantly higher than those group injected with naked plasmid (12.87%), plasmid-empty microspheres (19.72%) and saline (58.58%) respectively. PCR and RT-PCR showed that the expression level of GRF gene on the group injected with pcDNA3-GRF (1-32) microspheres was the highest. GRF gene released by microspheres was still detected after 30 days. In conclusion, pcDNA3-GRF (1-32) microspheres have a controlled release effect and GRF gene could be successfully transfected into muscle cells of mouse by microspheres with higher efficacy and stronger biological function.
