Preliminary study of ALK3 downstream genes related to ventricular septum defect.
- Author:
De-Ye YANG
1
;
Hou-Yan SONG
;
Huai-Qin ZHANG
;
Xiao-Yan HUANG
;
Xiao-Qun GUAN
Author Information
1. Department of Cardiology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China. Deyeyang@hotmail.com
- Publication Type:Journal Article
- MeSH:
1-Alkyl-2-acetylglycerophosphocholine Esterase;
genetics;
metabolism;
14-3-3 Proteins;
genetics;
metabolism;
Animals;
Bone Morphogenetic Protein Receptors, Type I;
genetics;
metabolism;
Genotype;
Heart Septal Defects, Ventricular;
genetics;
Mice;
Mice, Knockout;
Oligonucleotide Array Sequence Analysis;
PAX8 Transcription Factor;
Paired Box Transcription Factors;
genetics;
metabolism;
Protein-Tyrosine Kinases;
genetics;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Signal Transduction;
genetics;
physiology
- From:
Chinese Journal of Biotechnology
2003;19(3):267-271
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the function of ALK3 gene, the gene regulation and the signaling pathway related to ventricular septum defect during heart development. The model mice with ALK3 gene knock-out via alpha-MHC-Cre/lox P system were bred. The mRNA expression level of control group was compared with that of experiment group and ALK3 downstream genes were screened using PCR-select cDNA subtraction microarray. The mRNA of control group was extracted from E11.5 normal mouse hearts, and that of experiment group, from E11.5 hearts of mice with alpha-MHC Cre(+/-) ALK3(F/+) genotype. It was found that the mice with ALK3 gene knock-out produced heart defects involving the interventricular septum. The platelet-activating factors acetylhydrolase and the transcription factor Pax-8 and so on, were down-regulated. However, the Protein Tyrosine Kinase (PTK) of Focal Adhesion Kinase (FAK) subfamily and beta subtype protein 14-3-3 were up-regulated in the alpha-MHC Cre(+/-) ALK3(F/-) mice. These data provide support that ALK3 gene played an important role during heart development. The platelet-activating factors acetylhydrolase and Pax-8 genes could be important ALK3 downstream genes in the BMP signaling pathway during interventricular septum development. PTK and beta subtype protein 14-3-3 might be regulatory factors in this pathway.
