Study on the culture of crown gall from Panax quinquefolium and the prouction of its secondary metabolites--Ginsenosides Re and Rg1.
- Author:
Rong-Min YU
1
;
Yong-Bo SONG
;
Hui ZHANG
;
Wen-Cai YE
;
Yin-Lin ZHANG
;
Xin-Sheng YAO
Author Information
1. College of Pharmacy, Jinan University, Guangzhou 510632, China. rongminyu99@hotmail.com
- Publication Type:Journal Article
- MeSH:
Agrobacterium tumefaciens;
genetics;
physiology;
Cell Culture Techniques;
Ginsenosides;
biosynthesis;
Hydrogen-Ion Concentration;
Panax;
growth & development;
metabolism;
microbiology;
Plant Tumors;
microbiology
- From:
Chinese Journal of Biotechnology
2003;19(3):372-375
- CountryChina
- Language:Chinese
-
Abstract:
It was clearly demonstrated that T-DNA of Agrobacterium tumefeciens Ti plasmid was integrated into the cells of crown gall in our experiment. This paper reported the influences of some kinds of physical-chemistry factors on the growth of crown gall of Panax quinquefolium and the production of its main active compounds--ginsenoside Re and ginsensoside Rg1. The results showed that White medium was the best one for ginsensoside Rg1 accumulation (0.095%) among the six media, but ginsensoside Re accumulation (0.194%) was the highest on the MS medium; The highest contents of ginsensoside Re (0.147%) and ginsensoside Rg1 (0.061%) were on the culture 36d and 32d after innoculum respectively; The optimum pH was 5.6 for ginsensoside Rg1 synthesis(0.054%), and 5.8 for ginsensoside Re synthesis(0. 184% ); The contents of ginsensoside Re and ginsensoside Rg1 was the highest in the inoculum of 4 g and 2 g/flask (FW) respectively. The result also indicated that the concentration of inositol in 0.05 g/L could obviously promote ginsensoside Re synthesis (0.182%), and in 0.30 g/L for ginsensoside Rg1 (0.055%).
