Isolation and identification of reprogramming genes related to the development of the rabbit reconstructed embryos.
- Author:
Wei-Dong YU
1
;
Wen-Yong LI
;
Yu-Ge WANG
;
Li-Xin YANG
;
Gui-Sheng LIU
;
Miao DU
;
Qing-Xuan CHEN
Author Information
1. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Graduated School of Chinese Academy of Sciences, Beijing 100080, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Blastocyst;
metabolism;
physiology;
Blotting, Northern;
Embryo, Mammalian;
metabolism;
Embryonic Development;
genetics;
physiology;
Female;
Gene Expression Regulation, Developmental;
genetics;
physiology;
Polymerase Chain Reaction;
Pregnancy;
Rabbits
- From:
Chinese Journal of Biotechnology
2003;19(1):30-34
- CountryChina
- Language:Chinese
-
Abstract:
By the method of single preimplantation embryos differential display polymerase chain reaction (SPEDDRT-PCR), 25 reprogramming cDNA fragments were obtained from single 2-cell, 8-cell embryos and blastula. After cloning and sequencing, five of them were identified by reverse-Northern and characterized with stage-specific expression during reconstructed embryo development. This results will help to isolate full length reprogramming genes and study their function during embryonic development.
