Construction and biological activity study of human osteoprotegerin expressing adenoviral system.
- Author:
Ji-Zhong LIU
1
;
Zong-Ling JI
;
Yun-Yu HU
;
Su-Min CHEN
;
Bang-Fu ZHU
;
Tong-Tao YANG
Author Information
1. The Fourth Military Medical University, Department of Orthopedics, Xijing Hospital, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
Animals;
Blotting, Western;
Cell Line;
Enzyme-Linked Immunosorbent Assay;
Genetic Vectors;
genetics;
Humans;
Male;
Mice;
Mice, Inbred BALB C;
Osteoclasts;
metabolism;
Osteoprotegerin;
genetics;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Chinese Journal of Biotechnology
2003;19(1):35-40
- CountryChina
- Language:Chinese
-
Abstract:
Using the isolated total RNA from osteosacoma cell line MG63, the cDNA encoding human OPG was amplified by RT-PCR. A recombinant adenoviral vector carrying cDNA of OPG was constructed and OPG expression in mouse myoblast C2C12 cells was confirmed by Western blot and ELISA. The secreted expression of OPG protein persisted more than 6 weeks in vitro, and the growth of C2C12 cells infected by recombinant adenoviral were in good state. Osteoclasts derived from mouse bone marrow cells infected with recombinant adenoviral made less number of TRAP positive cells and resorption pits formed on dentine slices.
