Effect of qingfei oral liquid on protein expression of transforming growth factor-beta1 and platelet derived growth factor-BB of adenovirus type 3I, 7b induced human embryonic lung fibroblast cells.
- Author:
Wen-ge WANG
1
;
Si-wen CHEN
;
Shou-chuan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviruses, Human; classification; drug effects; growth & development; Animals; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Embryo, Mammalian; Female; Fibroblasts; cytology; virology; Humans; Male; Platelet-Derived Growth Factor; biosynthesis; Proto-Oncogene Proteins c-sis; Rabbits; Transforming Growth Factor beta; biosynthesis; Transforming Growth Factor beta1
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(7):643-645
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of qingfei oral liquid (QOL) contained serum on protein expression of transforming growth factor-beta1 (TGF-beta1) and platelet derived growth factor-BB (PDGF-BB) of adenovirus type 3I, 7b induced human embryonic lung fibroblast cells.
METHODSThe cells were divided into 5 groups, the normal cells group (NCG), the virus control group (VCG), the blank serum group (BSG), the ribavirin group (RVG) and the QOL contained serum group (QSG). All the cells except those in the NCG were challenged by adenovirus type 3I, 7b and treated with correspondent medicine. The contents of TGF-beta1 and PDGF-BB in the supernatant of cell culture were monitored by ELISA and compared among groups.
RESULTSContents of TGF-beta1 and PDGF-BB in VCG were significantly higher, while those in QSG were significantly lower than those in VCG (P < 0.01).
CONCLUSIONAdenovirus infection can increase the protein expression of TGF-beta1 and PDGF-BB of human embryonic lung fibroblast cells. QOL can decrease the protein expression of these cytokines, which maybe one of the mechanisms of its antiviral effect.
