Effect of astragalus injection on left ventricular remodeling and apoptotic gene caspase-3 in rats after myocardial infarction.
- Author:
Lan MA
1
;
Zhen-zhong GUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Astragalus membranaceus; Caspase 3; Caspases; biosynthesis; genetics; Down-Regulation; Drugs, Chinese Herbal; pharmacology; Injections; Male; Myocardial Infarction; metabolism; pathology; physiopathology; Myocardium; ultrastructure; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Wistar; Ventricular Remodeling; drug effects
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(7):646-649
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Astragalus injection (AI) on left ventricular remodeling and the expression of apoptotic gene caspase-3 in rats after myocardial infarction (MI).
METHODSThe MI model was established. The experimental animals were divided into 4 groups, Group I (the sham-operated group), Group II (the sham-operated plus AI group), Group III (the model group), Group IV (the model plus AI group). Animals in the II and IV group were intraperitoneally injected with 2 ml AI once a day after operation, while animals in the I and III group were treated with normal saline of equal volume. After treated for 4 weeks successively, the structural change of left ventricle and the level of oxyproline in myocardium were observed, and expression of caspase-3 was determined by immunohistochemical method.
RESULTSAs compared with Group Ill, the ultrastructure of myocardium and indexes of left ventricular remodeling were improved, the myocardial content of oxyproline decreased (P < 0.05), the caspase-3 positive cells reduced and caspase-3 mRNA expression significantly down-regulated (P < 0.05).
CONCLUSIONAI can improve left ventricular remodeling, inhibit apoptosis by down-regulate the expression of apoptotic gene caspase-3 in rats after MI.
