Experimental study on effect of jinye baidu preparation in inhibiting human cytomegalovirus protein kinase pul 97.
- Author:
Hui YUAN
1
;
Liang-Zhen WEN
;
Dan LI
;
Jixiao LIU
Author Information
- Publication Type:Journal Article
- MeSH: Antiviral Agents; pharmacology; Cytomegalovirus; drug effects; enzymology; genetics; Drugs, Chinese Herbal; pharmacology; Embryo, Mammalian; Fibroblasts; cytology; virology; Humans; Lung; cytology; Protein Kinases; biosynthesis; drug effects; genetics; RNA, Messenger; biosynthesis; genetics; Viral Proteins; biosynthesis; drug effects; genetics; Virus Replication; drug effects
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(8):734-737
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibitory effect of Jinye Baidu Preparation (JBP), a Chinese medicinal preparation, on human cytomegalovirus protein kinase pu197 and to explore its molecular mechanism in treating human cytomegalovirus (HCMV) infection.
METHODSExpression of the HCMV pu197mRNA in infected cells was measured by semi-quantitative RT-PCR before and after intervention of JBP or Ganciclovir (GCV), and effect of the two medicines on the proliferation activity of the infected cells was observed by MTT.
RESULTSBoth JBP and GCV showed obvious inhibitory action on HCMV pu197mRNA. They could significantly enhance the proliferation activity of the cells 72 hours after HCMV infection.
CONCLUSIONJBP could inhibit the gene expression and duplication of HCMV by inhibiting the gene expression of HCMV protein kinase pu197 to enhance the proliferation activity of the infected cells so as to achieve its anti-virus action.
