Experimental study on effect of baoganning on activity of nuclear transcription factor-kappaB in hepatic stellate cells.
- Author:
Yan TAN
1
;
Zhi-ping LU
;
Su-neng OU
;
Xufu ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; DNA; drug effects; metabolism; Drugs, Chinese Herbal; pharmacology; Female; Hepatocytes; cytology; metabolism; Male; NF-kappa B; drug effects; genetics; metabolism; Phosphorylation; drug effects; Random Allocation; Rats; Rats, Wistar
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(9):804-807
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Baoganning (BGN) on activity of nuclear transcription factor-kappaB (NF-kappaB) in hepatic stellate cells (HSCs) and its relevant mechanisms.
METHODSNormal Wistar rats were medicated with BGN decoction by gavage for 7 days to prepare BGN drug-serum. The effect of BGN drug-serum on HSC-T6 growth was measured by MTT assay; phosphorylation level of NF-kappaB inhibiting factor IkappaB at different time after BGN stimulation was detected by Western blotting analysis; and the binding level of NF-kappaB with DNA was measured 30 min after drug-serum stimulation with gel shift assay.
RESULTSBGN could significantly inhibit the HSC-T6 growth and quickly supress the phosphorylation of IkappaB, with the effect reached its peak at 30 min and restored to baseline level 6 h after stimulation, and reduce the binding capacity of NF-kappaB with DNA.
CONCLUSIONBGN can inhibit phosphorylation of IkappaB, restrain the activity of NF-kappaB and change the binding level of NF-kappaB with DNA.
