Effect of Lianggesan on the expression of signal transducer and activator of transcription 1 in a rat model of lipopolysaccharide-induced acute lung injury.
- Author:
Lin-zhong YU
1
;
Jian-xin LIU
;
Kong-you HU
;
Jian-xin DIAO
;
Li WANG
Author Information
- Publication Type:Journal Article
- MeSH: Acute Lung Injury; chemically induced; drug therapy; metabolism; Animals; Drugs, Chinese Herbal; pharmacology; therapeutic use; Female; Lipopolysaccharides; Lung; metabolism; Random Allocation; Rats; Rats, Wistar; STAT1 Transcription Factor; genetics; metabolism
- From: Journal of Southern Medical University 2010;30(1):43-46
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Lianggesan on the expression of signal transducer and activator of transcription 1 (STAT1) in rats with lipopolysaccharide (LPS)-induced acute lung injury and explore the possible mechanisms of the therapeutic effects.
METHODSEndotoxemia was induced in Wistar rats by intravenous injection of LPS (5 mg/kg). The rats were randomly divided into 6 groups, namely the control group, acute lung injury group (LPS group), 3 Lianggesan groups treated at different doses, and LPS+DEX treatment group. Each group, except for the control group, was further divided into 5 subgroups and examined at 1, 2, 4, 8 and 16 h after LPS injection. Western blotting was used to detect the protein expression of STAT1 and p-STAT1 in the lung tissue.
RESULTSIn LPS group, the expression of STAT1 began to increase at 1 h following LPS injection, reaching the peak level at 4 h; the peak expression of p-STAT1 occurred at 2 h after LPS administration (P<0.01). Compared with LPS group, the 3 Lianggesan groups and DEX group showed significantly decreased expressions of STAT1 and p-STAT1 at 2, 4 and 8 h after LPS injection (P<0.05 or 0.01).
CONCLUSIONAbnormal expression of STAT1 occurs in the lung tissue in the event of ALI. Lianggesan can relieve LPS-induced acute lung injury in rats by decreasing the expression of STAT1 and p-STAT1.
