Protective effect of mild hypothermia on astrocytes with traumatic or ischemic injury.
- Author:
Qiong CAO
1
;
Lan-lan ZHANG
;
Zi-you HU
;
Bing-yi WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Astrocytes; enzymology; pathology; Brain Injuries; therapy; Brain Ischemia; therapy; Cell Hypoxia; Cells, Cultured; Cold Temperature; L-Lactate Dehydrogenase; metabolism; Male; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2010;30(1):61-63
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of mild hypothermia on rat astrocytes with traumatic or ischemic injury.
METHODSRat astrocytes in primary culture were subjected to scratching or hypoxic injury and exposed to normothermia (37 degrees celsius;) or hypothermia (34 or 32 degrees celsius;) for 24 h. The morphology of the astrocytes was evaluated by live/dead staining, and the cell injury was measured by lactate dehydrogenase (LDH) release assay.
RESULTSAs the temperature reduced the LDH release rate from the cells in hypoxic group decreased significantly, to (11.48 - or + 1.53)% at 34 degrees celsius; and (3.79 - or + 0.45)% at 32 degrees celsius; as compared to that in normothermia [(33.02 - or + 3.58)%] in the absence of rat white blood cells (WBC) (P<0.001). LDH release rate of the hypoxic cells further decreased in the presence of rat WBC to (51.14 - or + 2.17 )% at 37 degrees celsius;, (19.53 - or + 4.37)% at 34 degrees celsius; and (16.68 - or + 1.47)% at 32 degrees celsius; (P<0.001). In the scratched cells, with or without WBC, LDH release rate showed no significant variation between the 3 temperatures (P>0.05).
CONCLUSIONMild hypothermia offers obvious protective effects on rat astrocytes against ischemic damage but not against mechanical injury.