Detection of Helicobacter species related genes coding for 16S rRNA in the liver tissue of patients with chronic liver disease.
- Author:
Ren CHEN
1
;
Xiao-ping CHEN
;
Qiu-qiong LIN
;
Bing-liang LIN
;
Hui-juan CAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Base Sequence; Female; Helicobacter Infections; complications; diagnosis; microbiology; Helicobacter pylori; genetics; isolation & purification; Hepatitis, Chronic; complications; microbiology; Humans; Liver Cirrhosis; complications; microbiology; Liver Neoplasms; complications; microbiology; Male; Middle Aged; Molecular Sequence Data; RNA, Bacterial; analysis; RNA, Ribosomal, 16S; analysis; Young Adult
- From: Journal of Southern Medical University 2010;30(1):131-132
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the infection of Helicobacter pylori (Hp) in the liver tissue of patients with chronic liver disease and the association between Hp and chronic liver disease.
METHODSLiver tissue samples were obtained by liver biopsy and surgical resection from 30 healthy subjects, 30 patients with chronic hepatitis, 30 with cirrhosis and 30 with liver cancer. All the samples were confirmed by pathological examination. The gene fragment coding for 16SrRNA were amplified by PCR with sequence analysis.
RESULTSThe PCR product of the 16SrRNA gene was 109 bp in length. Hp 16SrRNA was detected in 18 out of 30 liver biopsy samples from patients with primary cancer (60.0%), in 14 samples from patients with liver cirrhosis (47.0%), and in none of the samples from normal subjects or patients with chronic hepatitis. Sequencing analysis of Hp 16SrRNA gene in the liver tissue showed a 98.8% homology with the gene fragment encoding Hp 16SrRNA.
CONCLUSIONHp is identified in the liver tissue of patients with chronic liver disease, suggesting the possible correlation between Hp infection and hepatocellular carcinoma.
