High-performance liquid chromatography-based determination of plasma and renal tissue cisplatin levels after subcutaneous cisplatin implantation in mice.
- Author:
Wei-feng LI
1
;
Xiao-feng NIU
;
Te FAN
;
Xiao-peng WANG
;
Yan-ling LIU
;
Tian-ning CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; administration & dosage; blood; pharmacokinetics; Chromatography, High Pressure Liquid; Cisplatin; administration & dosage; blood; pharmacokinetics; Injections, Subcutaneous; Kidney; metabolism; Male; Mice
- From: Journal of Southern Medical University 2010;30(2):242-244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for determining plasma and renal tissue concentrations of cisplatin (DDP) after subcutaneous DDP implantation in mice.
METHODSDDP was extracted from the plasma and tissue of mice receiving subcutaneous DDP implantation and reacted with sodium diethyldithiocarbamate (DDTC). The product Pt (DDTC)(2) extracted by diethyl ether was determined using high-performance liquid chromatography (HPLC) with the mobile phase of water and methanol at the ratio of 25:75 and the flow rate of 1.0 ml/min. The derivatives of DDP and nickel chloride were detected at the wavelength of 254 nm.
RESULTSThe linear range of DDP was 0.1-10 microg/ml (r=0.9998 for plasma and 0.9993 for kidney). The intra-day and inter-day RSD was below 10%, and the minimum concentration detectable was 50 ng.
CONCLUSIONThe method is accurate and effective for determining plasma and tissue DDP levels after subcutaneous DDP administration and can be used in pharmacokinetic study of DDP.
