Effect of adenovirus-mediated CD/TK double suicide gene system on colorectal cancer growth and cytokines in the tumor microenvironment in mice.
- Author:
Xiao-jun YAO
1
;
Zong-hai HUANG
;
Qiang LI
;
Zhao-yang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; metabolism; Animals; Cell Proliferation; Colorectal Neoplasms; metabolism; pathology; therapy; Cytokines; metabolism; Cytosine Deaminase; genetics; metabolism; Female; Genes, Transgenic, Suicide; genetics; Genetic Therapy; methods; Green Fluorescent Proteins; genetics; metabolism; Interleukin-2; metabolism; Male; Mice; Mice, Inbred BALB C; Neoplasm Transplantation; Random Allocation; Recombinant Fusion Proteins; genetics; metabolism; Thymidine Kinase; genetics; metabolism; Tumor Necrosis Factor-alpha; metabolism; Vascular Endothelial Growth Factor A; genetics; metabolism
- From: Journal of Southern Medical University 2010;30(2):260-262
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of adenovirus-mediated CD/TK double suicide gene system on tumor growth and cytokine levels in the tumor microenvironment in mice bearing transplanted colorectal cancer.
METHODSCT26 cells were implanted subcutaneously into 30 Balb/c mice, which were subsequently randomized into the control (n=15) and experimental group (n=15). After the tumor formation, CD/TK double suicide gene system was administered for tumor treatment, and the changes in the tumor volume, tumor inhibition rate, and levels of cytokines in the tumor microenvironment were investigated.
RESULTSCD/TK double suicide gene system resulted in a significant inhibition of the tumor growth and significantly increased levels of such cytokines as IL-2, IL-10, TNFalpha and IFNgamma in the tumor microenvironment.
CONCLUSIONCD/TK double suicide gene system produces significant tumor inhibition effect and causes obvious cytokine changes in the tumor microenvironment in mice bearing transplanted colorectal cancer.
