Establishment of a cell culture model of corpus cavernosum smooth muscle cells derived from diabetic rats with erectile dysfunction.
- Author:
Ting-yu YE
1
;
An-yang WEI
;
Bo WAN
;
Yong YANG
;
Xin-gui LUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Culture Techniques; Cells, Cultured; Diabetes Mellitus, Experimental; complications; pathology; physiopathology; Erectile Dysfunction; etiology; pathology; physiopathology; Male; Models, Biological; Myocytes, Smooth Muscle; cytology; physiology; Penile Erection; Penis; cytology; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2010;30(2):351-354
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the method for culturing corpus cavernosum smooth muscle cells (CCSMs) derived from diabetic rats with erectile dysfunction (ED) for the study of ED caused by diabetes.
METHODSCCSMs were isolated from the corpus cavernosum of diabetic rats with ED and cultured using a modified method of adherent tissue culture. The cultured cells were identified by immunohistochemistry and the cell morphology and proliferation were observed.
RESULTSThe primary culture of CCSM was performed successfully, and the cells were seen to migrate from the small tissue pieces 3 days later, reaching nearly confluence in 16-18 days. A typical "hill-valley" growth pattern was noted in the cell passaging. Immunohistochemical staining for alpha-smooth muscle actin (alpha-SM-actin) and desmin yielded positive results in the cells.
CONCLUSIONThe modified method for adherent tissue culture is convenient and reliable in establishing the in vitro cell culture model of CCSMs from diabetic rats with ED, and the cultured CCSMs display a faster proliferation than normal CCSMs. No obvious differences in the cell morphology can be found between diabetic and normal CCSMs under light microscope.
