Signal transduction pathway in liver fibrosis regulated by human hepatocyte growth factor.
- Author:
Hao LIU
1
;
Jing-liang CHEN
;
Guo-an XIANG
Author Information
- Publication Type:Journal Article
- MeSH: Gene Expression Profiling; Gene Expression Regulation; Hep G2 Cells; Hepatocyte Growth Factor; genetics; metabolism; Humans; Janus Kinases; metabolism; Liver Cirrhosis; genetics; metabolism; Mitogen-Activated Protein Kinase 1; metabolism; STAT1 Transcription Factor; metabolism; Signal Transduction; physiology; Transfection
- From: Journal of Southern Medical University 2010;30(3):431-434
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the changes in the gene expression profiles in HepG2 cells transfected by human hepatocyte growth factor (hHGF) and analyze the signal transduction pathway in liver fibrosis regulated by hHGF.
METHODA 20,000 gene cDNA microarray (Affymetrix) was used to examine the gene expressions in the HepG2 cells transfected by hHGF. The differentially expressed genes were identified and some genes with possible contribution to hepatic fibrosis were subjected to real-time PCR analysis.
RESULTThe differentially expressed genes were mostly transcription regulatory molecules, cytokines, signal transduction, glucose metabolism, lipid metabolism. The results of real-time PCR showed up-regulated STAT1 and MAPK1 expression in the cells as were consistent with genechip analysis results.
CONCLUSIONhHGF gene transfection results in the gene expression profile changes in HepG2 cells. HGF may regulate liver fibrosis via the JAK/STAT and MAPK pathways.
