Genotype of human calicivirus from infants with diarrhea in Guangzhou city in 2001.
- Author:
Jian-ping XIE
1
;
Zhao-yin FANG
;
Si-tang GONG
;
Bing ZHU
;
Wen-ji OU
;
Gen-ping WAN
;
Gui-xiang LAI
;
Cui-juan HE
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Caliciviridae; genetics; Caliciviridae Infections; complications; virology; China; DNA, Viral; chemistry; genetics; Diarrhea, Infantile; etiology; Dysentery; etiology; Feces; virology; Genotype; Humans; Infant; Molecular Sequence Data; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid
- From: Chinese Journal of Pediatrics 2003;41(11):842-844
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo understand the characteristics of human calicivirus (HuCV) infection in infants with diarrhea in Guangzhou city and to study genotype of the virus.
METHODSThe authors collected fecal specimens from 22 children with acute nonbacterial gastroenteritis from November to December, 2001. HuCV was detected from the specimens by RT-PCR. The PCR products were cloned into the PMD18-T cloning vector and sequenced.
RESULTSHCV was detected from the specimens of 2 cases (9%, 2/22). The nucleotide sequence analysis revealed that the virus strains belonged to genotype 2 of Norwalk-like viruses.
CONCLUSIONHuCV is one of the pathogens causing diarrhea in infants and young children in Guangzhou area. HuCV infection occurred sporadically in autumn and winter.
