Automated ribotyping of Salmonella and Staphylococcus aureus in food poisoning of Guangdong province
10.3760/cma.j.issn.0254-6450.2011.09.016
- VernacularTitle:广东省食物中毒暴发疫情中沙门菌和金黄色葡萄球菌自动化核糖体基因分型研究
- Author:
Dong-Mei HE
1
;
Hai-Ming ZHU
;
Wei-Dong LAI
;
Hai-Yan WANG
;
Bi-Xia KE
;
Hai-Ling TAN
;
Bo-Sheng LI
;
Xiao-Ling DENG
;
Chang-Wen KE
Author Information
1. 广东省疾病预防控制中心
- Keywords:
Salmonella;
Staphylococcus aureus;
Ribotyping
- From:
Chinese Journal of Epidemiology
2011;32(9):918-923
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the genetic polymorphism of Salmonella and Staphylococcus aureus in Guangdong province, as well as to explore methods for identifying and tracing the source of these two foodbome pathogens. Methods Using the automated ribotyping system, two foodbome pathogens were tested with either EcoR Ⅰ or Pvu Ⅱ restriction enzymes. BioNumerics software was then applied for image analysis, database establishment and other corresponding analysis. Results Digestion of 32 Salmonella isolates with Pvu Ⅱ yielded 19 different ribotypes,and digestion of 14 Salmonella isolates with EcoR Ⅰ yielded 2 different ribotypes. Staphyloccus aureus isolates showed greater genetic diversity, whereas EcoR Ⅰ digestion of 49 different isolates yielded 31 different ribotypes. Conclusion Unique Salmonella and Staphylococcus aureus isolates could be identified through ribotyping. Although Salmonella serotyping and ribotyping were not strongly correlated, the combination of both restriction enzymes could be used to more effectively identify the genetic relationship among different strains as well as the source of food poisoning. Thus, not only could the genetic relationships amongst the different strains be inferred through ribotyping skills, the source of food poisoning and mode of transmission could also be determined under the use of this method.
