Study on the molecular-epidemiological characteristics of HIV-1 in Shenzhen, 1992- 2008
10.3760/cma.j.issn.0254-6450.2012.01.019
- VernacularTitle:深圳地区1992-2008年HIV-1分子流行病学研究
- Author:
Guang-Lu ZHAO
1
;
Wei YU
;
Juan-Juan ZHANG
;
Lin CHEN
;
Tie-Jian FENG
;
Feng WANG
;
Fu-Chang HONG
;
Xiao-Hui WANG
;
Qing LI
Author Information
1. 深圳市慢性病防治中心
- Keywords:
Human immunodeficiency virus-1;
Molecular epidemiolog
- From:
Chinese Journal of Epidemiology
2012;33(1):82-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the epidemiological characteristics of HW-1 subtype in Shenzhen from 1992 to 2008.Methods 489 HIV-1 positive plasma samples were collected from 1992 to 2008 in Shenzhen.HIV-1 env genes were amplified by nested-PCR from RNA.Phylogenetic analysis was performed on data regarding the nucleotide sequence.Results A total of 464 sequences were amplified and genotyped.Data from this study revealed that CRF01_AE was a predominant HIV-1 subtype in Shenzhen (64.4％,299/464),followed by subtypes CRF_BC( 17.5％,81/464),B'( 14.7％,68/464) and B (2.4％,11/464).Subtype C (0.4％,2/464),A1 (0.2％,1/464),CRF02_AG(0.2％,1/464) and CRF06_cpx (0.2％,1/464) were also prevalent in Shenzhen.CRF01_AE and CRF_BC were predominant among heterosexuals,homosexuals and injection drug users,while B'was predominant among blood donors.Results from phylogenetic tree analysis showed that some of the HIV-1 clusters had been defined in CRF01_AE strains at different time or groups with different transmission routes.Cross-infections were also seen.Conclusion CRF01_AE was the predominant HIV-1 subtype in Shenzhen while CRF_BC,B,B',C,A1,CRF02_AG and a small amount of CRF06_cpx or recombinant subtypes were prevalent in this city.Different subtypes showed great variation in the process of epidemics.
