Genetic diversification of avian influenza H5N1 virus in boundary areas of Yunnan province
10.3760/cma.j.issn.0254-6450.2012.03.017
- VernacularTitle:云南省边境地区禽流感H5N1亚型病毒遗传多样性分析
- Author:
Wu-Lin ZHANG
1
;
Wen-Dong ZHANG
;
Huan-Yun ZHAO
;
Xiu-Hua QI
;
Ting-Song HU
;
Yue LV
;
Liang YUE
;
Wei QIU
;
Zi-Liang FENG
;
Ying ZHENG
;
Quan-Shui FAN
;
Ying-Guo ZHANG
;
Xing-Lin JIA
;
Fu-Qiang ZHANG
Author Information
1. 湖南农业大学
- Keywords:
Avian influenza virus;
H5N1 subtype;
Hemagglutinin;
Genetic diversification
- From:
Chinese Journal of Epidemiology
2012;33(3):323-327
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the genetic diversifications of avian influenza subtype H5N1 viruses in the boundary regions of Yunnan province during 2009 to July,2011.Methods Swab samples were collected from foreign poultry and wild birds in boundary regions of Yunnan province during 2009 to July,2011 and tested by H5/N1 subtype-specific multiplex RT-PCR.The HA genes of H5N1 virus from the positive samples were amplified by RT-PCR and cloned into pMD 18-T vectors for sequencing.Both alignment and phylogenetic analysis were performed with sequences of the known reference strains.Results Fifteen different HA sequences were obtained from 36 representative positive samples and could be divided into 2 distinct Clades (2.3.2 and 2.3.4).Through phylogenetic analysis,Clade 2.3.2 and 2.3.4 could then be further divided into 3 ( Ⅱ -1 to Ⅱ -3 ) and 2 smaller clades ( Ⅰ -1 and Ⅰ -2),respectively.The viruses of Clade 2.3.2 Ⅱ -1 and Ⅱ-2 were new variant strains of H5N 1 virus.The cleavage sites of HA from positive samples all possessed molecular characterization of highly pathogenic avian influenza virus.Mutation of key amino acids had been found among receptor binding sites,potential glycosylation sites,neutralizing epitopes and others.Conclusion It seemed evident that the H5N1 subtype viruses showed genetic diversifications and had undergone the evolution progress of multi-clade (2.3.2,2.3.4) to single caide (2.3.2) in the boundary regions of Yunnan province,during 2009 to July,2011.
