Effect of Shenqi Fuzheng injection on fractalkine expression in lung tissue of rats with lipopolysaccharide-induced acute lung injury.
- Author:
Lei WANG
1
;
Gang WANG
;
Ting-qian LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chemokine CX3CL1; biosynthesis; genetics; Drugs, Chinese Herbal; administration & dosage; pharmacology; Enzyme-Linked Immunosorbent Assay; Gene Expression; drug effects; Injections; Lipopolysaccharides; Lung; drug effects; metabolism; pathology; Lung Diseases; blood; chemically induced; genetics; Male; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; Tumor Necrosis Factor-alpha; blood
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(1):55-59
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe dynamically the Fractalkine (FKN) expression in lung tissue of rats with lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the effect of Shenqi Fuzheng Injection (SFI) on it.
METHODSRat model of ALI was established by intravenous injection of 4 mg/kg of LPS. Forty-two Wistar rats were randomly divided into 7 groups, the normal group, the model group and the SFI group, the latter two were separated respectively into three time phases (the 1 h, 2 h and 4 h after modeling ) groups, 6 rats in each group. Pathological changes and wet/dry weight ratio (W/D) of lung were observed, serum TNF-alpha and FKN mRNA expression in the lung tissue were examined by ELISA and RT-PCR respectively.
RESULTSSevere pathological changes of lung presented in the model groups of all three time phases with a higher W/D ratio, as well as increased serum TNF-alpha level and FKN mRNA expression in lung tissue. The peak of abnormality of serum TNF-alpha level and FKN mRNA expression was shown in the 2 h time phase group. All the above-mentioned abnormal changes were alleviated after treatment in the SFI group (P<0.05). In addition, the level of FKN mRNA expression was found to be positively correlated to the serum TNF-alpha concentration.
CONCLUSIONSFI treatment in early stage could relieve the pathological changes and edema in lung tissue, decrease serum TNF-alpha and down-regulate FKN mRNA expression, playing a protective role in LPS-induced ALI rats.
