Study on effect of lentinan in enhancing anti-tumor action of dendritic cytoma vaccine and its mechanism.
- Author:
Jun WANG
1
;
Zhi-dong ZHOU
;
Da-jing XIA
Author Information
- Publication Type:Journal Article
- MeSH: Adjuvants, Immunologic; pharmacology; therapeutic use; Animals; Antineoplastic Agents, Phytogenic; pharmacology; therapeutic use; Cancer Vaccines; administration & dosage; immunology; Cell Line; Cell Line, Tumor; Combined Modality Therapy; Dendritic Cells; cytology; immunology; Female; Humans; Lentinan; pharmacology; therapeutic use; Melanoma, Experimental; immunology; pathology; therapy; Membrane Glycoproteins; genetics; immunology; Mice; Mice, Inbred C57BL; Random Allocation; Shiitake Mushrooms; chemistry; Survival Analysis; Transfection; gp100 Melanoma Antigen
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(1):60-64
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo improve the anti-tumor effect of dendritic cytoma vaccine (DCV) for finding an effective anti-tumor biotherapy.
METHODSDC vaccine prepared by transfection of adenovirus mediated melanoma-associated antigen gene (gp100) into bone marrow-derived dendritic cell (DC) was used to study the immuno-therapeutic effect and the mechanism of lentinan (LNT) in different dosages, used alone or combined with gp100-DC for treatment of B16 melanoma bearing mice.
RESULTSAfter being treated with LNT combining gp100-DC, the growth of malignant melanoma was inhibited with the tumor-free survival in the experimental animals being 66.7%. The treatment could also significantly enhance the activity of cytotoxicity T lymphocyte (CTL) and natural killer (NK) cells, elevate the levels of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) in splenocytes, and histological examination showed that a large amount of inflammatory cells infiltrated inside and around the tumor, and obvious necrosis of tumor cells was found.
CONCLUSIONBy combined use with LNT the anti-tumor immuno-reaction of DCV vaccine could be enhanced effectively.
