Establishment of oxygen and glucose deprive model of in vitro cultured hippocampal neuron and effect of ligustrazine on intracellular Ca+ level in model neurons.
- Author:
Hai-tong WAN
1
;
Yu WANG
;
Jie-hong YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Calcium; metabolism; Cell Hypoxia; Cells, Cultured; Glucose; metabolism; Hippocampus; cytology; Models, Biological; Neurons; cytology; drug effects; metabolism; Neuroprotective Agents; pharmacology; Oxygen; metabolism; Pyrazines; pharmacology; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(3):234-236
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the oxygen and glucose deprive (OGD) model in cultured hippocampal neuron and study the effect of ligustrazine on intracellular Ca2+ level in the model neurons.
METHODSThe OGD model was established in cultured hippocampal neuron, and the intracellular Ca2+ level in it was detected by laser scanning confocal microscope (LSCM).
RESULTSThe OGD model was successfully established in cultured hippocampal neurons; the intracellular Ca2+ level in the OGD model group was significantly higher than that in the blank control group (P < 0.05), and that in the nemodipine and high and medium dosage of ligustrazine treated groups was lower than that in the OGD model group (P < 0.05).
CONCLUSIONIntracellular Ca2+ overload occurs in OGD model neuron, which could be antagonized by ligustrazine, indicating that ligustrazine has a protective effect on hippocampal neuron from hypoxic-ischemic injury.
