Modulation effect of naomaitong on gelatinase system after cerebral ischemia/reperfusion in rats LI.
- Author:
Jian-sheng LI
1
;
Ke LIU
;
Ming-hang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Basement Membrane; drug effects; metabolism; Brain Ischemia; enzymology; Disease Models, Animal; Drugs, Chinese Herbal; pharmacology; Gelatinases; metabolism; Matrix Metalloproteinase 2; metabolism; Matrix Metalloproteinase 9; metabolism; Medicine, Chinese Traditional; Microvessels; drug effects; metabolism; Protective Agents; pharmacology; Rats; Reperfusion Injury; enzymology; Tissue Inhibitor of Metalloproteinase-1; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2006;26 Suppl():14-17
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the modulation effect of Naomaitong on gelatinase system after cerebral ische-Focal cerebral I/R rat model was duplicated by method of the intralumimia/reperfusion (I/R) in rats.
METHODnal filament technique. Rats were randomly divided into the sham-operative group, the model group, the Naomaitong group and the Nimodipine group, the latter three groups were also divided into the 3 hrs after ischemia group, and 6 hrs, 12 hrs, 24 hrs, 3 d, 6 d after IR groups. Immunohistochemical method and zymogram analysis method, etc. were adopted to observe the change of microvessel structure, gelatinase and its inhibitor expression.
RESULTSMMP-2 (IR 24 h-6 d)and MMP-9 (I/R 12 h-3 d) expression levels could be lowered and TIMP-1 expression level (IR 24 h-6 d) improved by Naomaitong. Besides, comparison of MMP-2 and MMP-9 content in zymogram analysis in each group showed that changes of its quantity were in accordance with the laws of immune expression.
CONCLUSIONThe protective effect of Naomaitong on cerebromicrovessel basement membrane injury in rats is related to its modulation on gelatinase system.
