Screening and identification of peptides specifically binding to human osteosarcoma cells.
- Author:
Kun LU
1
;
Yong JIANG
;
Mingqiang GUAN
;
Jun XIAO
;
Jian WANG
;
Zhihan LI
;
Zhanjun SHI
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Bone Neoplasms; drug therapy; Cell Line, Tumor; Drug Screening Assays, Antitumor; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Osteosarcoma; drug therapy; Peptide Library; Peptides; analysis; Protein Binding
- From: Journal of Southern Medical University 2012;32(5):647-650
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo obtain the peptide that specifically binds to human osteosarcoma MG-63 cells from Ph. D. 7TM phage display peptide library.
METHODSHuman osteosarcoma MG-63 cells were used as the target cells with human embryonic kidney 293T cells as the control for screening the peptide from Ph. D. 7TM phage display peptide library. The enriched specially binding peptides were verified by cell enzyme-linked immunosorbent assay (ELISA). The location of the peptide in MG-63 cells was investigated using cell fluorescence staining, and targeting of the peptide was tested by organ immunohistochemistry with Osteosarcoma model.
RESULTSThe specifically binding peptides were enriched after 4 rounds of screening. The sequence SLTNLSK was confirmed as the most frequent peptide by DNA sequencing and showed strong specificity verified by cell ELISA, fluorescent staining and organ immunohistochemistry.
CONCLUSIONA peptide that specifically binds to MG-63 cells has been screened from Ph. D. 7TM phage display peptide library to serve as a potential candidate for osteosarcoma-targeting therapy.
