Cytogenetic differences between adults and children with acute lymphoblastic leukemia: eight-probe fluorescence in situ hybridization and karyotype analyses.
- Author:
Yuan ZUO
1
;
Qingfeng DU
;
Rong LI
;
Na XU
;
Rui CAO
;
Libin LIAO
;
Lulu XU
;
Jinfang ZHANG
;
Bintao HUANG
;
Xujing LUO
;
Xiaozhen XIAO
;
Xiaoli LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Child; Child, Preschool; Cytogenetics; Female; Humans; In Situ Hybridization, Fluorescence; methods; Infant; Karyotype; Karyotyping; Male; Middle Aged; Precursor Cell Lymphoblastic Leukemia-Lymphoma; diagnosis; genetics; Young Adult
- From: Journal of Southern Medical University 2012;32(5):707-709
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the cytogenetic differences between children and adults with acute lymphoblastic leukemia (ALL) using eight-probe fluorescence in situ hybridization and karyotype analysis.
METHODSEight-probe (MYC, P16, E2A, TEL/AML1, BCR/ABL , MLL , IGH, and hyperdiploidy) fluorescence in situ hybridization and karyotype analysis were performed for 86 adults and 39 children with acute lymphoblastic leukemia.
RESULTSEight-probe fluorescence in situ hybridization showed significant differences in the positivity rate of TEL/AML1, BCR/ABL, and hyperdiploidy between adult patients and children with ALL. By karyotype analysis, the positivity rate of t(9;22) and hyperdiploidy differed significantly between the children and adult patients (P<0.05).
CONCLUSIONAdults and children with ALL have different expression profiles of the fusion genes. Eight-probe fluorescence in situ hybridization is time-saving, accurate and efficient in detecting common genetic abnormalities in ALL patients, and can be well complementary to karyotype analysis in clinical diagnosis of ALL.
