Effects of curcumin on syndecan-4 protein and p44/42 mitogen-activated protein kinase expression in tumor necrosis factor-α-induced rat vascular smooth muscle cells in vitro.
- Author:
Ye LUO
1
;
Ping OUYANG
;
Wenyan LAI
;
Dingli XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Curcumin; pharmacology; Mitogen-Activated Protein Kinase 3; metabolism; Muscle, Smooth, Vascular; drug effects; metabolism; Phosphorylation; drug effects; Rats; Rats, Sprague-Dawley; Syndecan-4; metabolism; Tumor Necrosis Factor-alpha; pharmacology
- From: Journal of Southern Medical University 2012;32(5):722-725
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of curcumin on the expression of syndecan-4 protein and p44/42 mitogen- activated protein kinase(MAPK) phosphorylation in rat vascular smooth muscle cells (VSMCs) induced by tumor necrosis factor-α (TNF-α) in vitro.
METHODSRat VSMCs cultured in vitro were stimulated for 24 h by 20 ng/ml TNF-α, 20 µmol/L curcumin, or 20 ng/ml TNF-α plus 20 µmol/lL curcumin. /assay was adopted to evaluate the proliferation of the VSMCs, and the expression of syndecan-4 protein and phosphorylated p44/42 MAPK were determined by Western blotting.
RESULTSCompared with the normal control cells, VSMCs exposed to TNF-α showed significantly enhanced proliferation (P/0.01). Curcumin treatment did not obviously affect the growth of otherwise untreated VSMCs(P>0.05), but could significantly suppress TNF-α-induced proliferation of VSMCs (P/0.01). TNF-α treatment also significantly increased the expression of syndecan-4 protein and phosphorylated p44/42 MAPK (P<0.01), which was markedly lowered by treatment with curcumin (P/0.01). Curcumin alone did not produce any obvious effects on the expression of syndecan-4 protein or phosphorylated p44/42 MAPK (P>0.05).
CONCLUSIONCurcumin can suppress the proliferation of rat VSMCs and lower the expression of syndecan-4 protein and phosphorylated p44/42 MAPK in TNF-α-induced VSMCs.
